TABLE 1.
Parameter estimations for plasma protein binding of NPX
| Parameters | Definition | Estimates (CV%) |
|||
|---|---|---|---|---|---|
| CIA Females | Healthy Females | CIA Males | Healthy Males | ||
| Ka1 (µM−1) | Association constant for first binding site | 0.28 (3.53) | 0.25 (3.33) | 0.26 (4.00) | 0.26 (1.50) |
| Ka2 (µM−1) | Association constant for second binding site | 0.0041 (4.2) | 0.0043 (4.35) | 0.0056 (11.75) | 0.0054 (2.65) |
| n1 | Number of first-class binding sites | 1 (Fixed) | |||
| n2 | Number of second-class binding sites | 4 (Fixed) | |||
| Pt (µM) | Measured albumin concentration | 347 (Fixed) | 550 (Fixed) | 282 (Fixed) | 422 (Fixed) |
| n1Pta (µM) | Binding capacity of first binding site | 347 | 550 | 282 | 422 |
| n2Pta (µM) | Binding capacity of second binding site | 1388 | 2200 | 1128 | 1688 |
a
Binding capacities were calculated as the product of ni × Pt.