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. 2017 Apr 12;40(5):723–725. doi: 10.2337/dc16-2127

Figure 2.

Figure 2

Comparison of insulitic islets from nPOD donor 6310 with multiple (GADA+IA-2A+) autoantibody–positive nPOD donor 6267 and GADA+ nPOD donor 6362 with new-onset type 1 diabetes. Representative images from donors 6310 (A–C), 6267 (D–F), and 6362 (G–I) are shown. Formalin-fixed, paraffin-embedded tissue sections (4 μm) from the pancreas head, body, and tail regions were stained by double immunohistochemistry for CD3 (brown) and glucagon (red). The insulitis found in donor 6310 was mild. The majority of infiltrating cells were found to be inside the donor 6310 islets (intrainsulitis, green arrow) (A). Lymphocytic infiltration was also observed in the donor 6310 islet periphery (peri-insulitis), showing focal aggregation (B) and direct contact with the peripheral islet cells (C). Intrainsulitis (D) was seen in the donor 6267 pancreas (green arrow), but the insulitic lesions with peri-insulitis (E and F) were more abundant. The insulitis seen in the pancreas of donor 6362 with recent-onset type 1 diabetes was more robust, with higher numbers of lymphocytes observed inside (G) and on the periphery (H and I) of many islets. Scale bars: 200 μm (all panels).