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. 2017 Apr 15;144(8):1484–1489. doi: 10.1242/dev.145235

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© 2017. Published by The Company of Biologists Ltd

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.

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Fig. 1. — Downregulation of InR, TOR and intracellular Ca²⁺ signaling pathways in NE cells impairs larval development on sucrose. (A) Schematic of assay protocol with representative examples of pupa and adult appearance. (B,C) Pupariation upon reduction of InR/TOR signaling in dimm-GAL4 cells on sucrose (B) or normal diet (C). (D,E) Pupariation upon reduction of intracellular Ca²⁺ signaling on sucrose (D) or normal diet (E). Regulators of the InR (orange), TOR (green), Alk (blue) or intracellular Ca²⁺ (purple) signaling. UAS controls are shown in Fig. S1. Bars with the same letter represent statistically indistinguishable groups (one-way ANOVA with post-hoc Tukey's test, P<0.05). n=6 batches of 25 larvae each. Data represent mean±s.e.m.