Fig. 2.
Dysregulated protein metabolism in the IP3R hypomorph itprku can be rescued by overexpression of either InR or IP3R in NE cells. (A) Pupariation of 65 h larvae transferred into media with varying amounts of yeast. Two-way ANOVA, ****P<0.0001. (B) Pupariation over time after transfer to normal food at 88 h. Inset shows representative pupa. Relative pupal volume is shown in Fig. S2C. (C,E) Temporal changes in protein/TAG ratio, normalized to weight, for different genotypes. n≥5. See also Fig. S2D-F. (D,F) Ilp5 transcript levels in larval CNS normalized to rp49. n=6. (G) Pupariation of itprku on sucrose diet upon overexpression of positive regulators of InR and TOR signaling (orange and green) or intracellular Ca2+ signaling (purple) in NE cells. See also Fig. S3D. (H) Protein/TAG ratios normalized to weight. See also Fig. S2D-F. n≥8. (I) Ilp5 transcript levels in larval CNS normalized to rp49. n=4. Statistics: C,D,F, unpaired t-test, *P<0.05, **P<0.01, ****P<0.0001; E,G,H,I, one-way ANOVA. Bars with the same letter represent statistically indistinguishable groups (one-way ANOVA with a post-hoc Tukey's test, P<0.05). Data represent mean±s.e.m. CS, Canton S; ns, not significant; Wand., wandering.