Table 1.
Channels examined for osmotic upshifts
| Mutated gene | Gene family | Number of turns/min for the 5th minute | p value | |||
|---|---|---|---|---|---|---|
| Wild-type control | Mutant | |||||
| 150 mOsm | 400 mOsm | 150 mOsm | 400 mOsm | |||
| aqp-1 | Aquaporin | 9.50 ± 1.20 (16) | 27.50 ± 1.79 (16) | 13.56 ± 2.48 (16) | 33.80 ± 3.09 (15) | 0.617 |
| aqp-2 | Aquaporin | 10.88 ± 2.67 (8) | 16.63 ± 3.68 (8) | 8.00 ± 2.03 (8) | 20.14 ± 4.98 (7) | 0.357 |
| aqp-3 | Aquaporin | 5.93 ± 0.91 (15) | 21.87 ± 1.67 (15) | 6.00 ± 1.04 (14) | 16.87 ± 2.25 (15) | 0.114 |
| aqp-4 | Aquaporin | 8.44 ± 0.93 (16) | 21.94 ± 1.87 (16) | 9.94 ± 1.40 (16) | 29.38 ± 3.15 (16) | 0.146 |
| aqp-6 | Aquaporin | 10.25 ± 1.80 (8) | 28.38 ± 2.04 (8) | 7.50 ± 1.38 (8) | 28.13 ± 3.70 (8) | 0.606 |
| aqp-7 | Aquaporin | 10.33 ± 1.54 (12) | 29.00 ± 1.62 (16) | 9.19 ± 1.68 (16) | 23.06 ± 1.68 (16) | 0.156 |
| aqp-8 | Aquaporin | 9.31 ± 1.76 (16) | 24.63 ± 2.40 (16) | 12.17 ± 1.40 (12) | 27.44 ± 2.93 (16) | 0.993 |
| aqp-9 | Aquaporin | 11.5 ± 1.24 (32) | 33.16 ± 1.44 (32) | 11.78 ± 1.31 (32) | 27.41 ± 1.35 (32) | 0.026 |
| aqp-10 | Aquaporin | 9.50 ± 1.20 (16) | 27.50 ± 1.79 (16) | 6.63 ± 0.80 (16) | 25.67 ± 1.67 (15) | 0.714 |
| aqp-11 | Aquaporin | 8.44 ± 0.93 (16) | 21.94 ± 1.87 (16) | 7.88 ± 0.74 (16) | 25.56 ± 2.22 (16) | 0.187 |
| asic-1 | Epithelial sodium channel (ENaC) | 13.25 ± 0.95 (36) | 29.49 ± 1.47 (37) | 14.74 ± 1.12 (35) | 32.05 ± 1.29 (39) | 0.664 |
| mec-4 | Epithelial sodium channel (ENaC) | 16.59 ± 1.48 (32) | 31.29 ± 1.58 (28) | 20.11 ± 2.05 (28) | 35.72 ± 1.57 (32) | 0.784 |
| trp-4 | Transient receptor potential (TRP) channel | 11.17 ± 1.02 (15) | 32.29 ± 2.06 (15) | 14.54 ± 1.93 (15) | 35.42 ± 1.91 (15) | 0.944 |
| tmc-1 | Transmembrane channel-like protein | 14.75 ± 1.09 (24) | 27.83 ± 1.89 (24) | 12.29 ± 1.20 (24) | 29.92 ± 1.39 (24) | 0.115 |
| tmc-2 | Transmembrane channel-like protein | 12.75 ± 1.29 (20) | 28.32 ± 2.15 (22) | 10.63 ± 0.98 (24) | 26.09 ± 1.96 (23) | 0.975 |
| tax-4 | Cyclic nucleotide-gated ion channel | 10.25 ± 1.15 (28) | 22.32 ± 1.35 (28) | 11.22 ± 1.13 (27) | 20.86 ± 1.12 (28) | 0.309 |
Values are reported as the mean ± SEM (n animals tested). Mutants with mutations in several channel-encoding genes are examined. The number of turns per minute is shown for the 5th minute for each genotype and osmolarity treatment. The interaction of genotype and treatment is tested with two-way ANOVA on the number of turns per minute in the 5th minute. The number of animals tested under each condition is indicated in the parenthesis next to mean ± SEM.