Table 3.
Genetic background | Transgene | Expressing neurons | Number of turns/min for the 5th minute Mean ± SEM (n) |
p value | |||
---|---|---|---|---|---|---|---|
Nontransgenic control | Transgenic animals | ||||||
150 mOsm | 400 mOsm | 150 mOsm | 400 mOsm | ||||
tax-2(p691) | Podr-1::tax-2 | AWB, AWC | 5.66 ± 0.69 (29) | 9.33 ± 0.89 (30) | 7.63 ± 1.15 (27) | 9.86 ± 1.11 (28) | 0.560 |
tax-2(p691) | Pstr-3::tax-2 | ASI | 6.84 ± 0.61 (31) | 12.31 ± 1.25 (32) | 8.55 ± 0.83 (31) | 17.14 ± 2.04 (29) | 0.565 |
Values are reported as the mean ± SEM (n animals tested). Transgenic animals that express a wild-type tax-2 cDNA in the major olfactory sensory neurons AWB and AWC (Podr-1::tax-2) or in the sensory neuron ASI (Pstr-3::tax-2) in the tax-2(p691) mutant background are examined for osmotic upshifts. The number of turns per minute is shown for the 5th minute for each genotype (transgenic vs nontransgenic siblings) and osmolarity treatment. The interaction of genotype and treatment is tested with two-way ANOVA on the number of turns per minute in the 5th minute. The number of animals tested under each condition is indicated in the parenthesis next to mean ± SEM.