Transport properties of human in-vitro matured oocytes. (A) Cell volume responses upon introduction of 0.56 M trehalose, 1.5 M EG, and 1.5 M PG at 4, 15, and 25 °C and the corresponding curve fitting results (lines) with the 2-p model of water/solute transport. (B) Arrhenius plots of the temperature-dependent membrane permeability coefficients (Lp and Ps). (C) The Lpg and Psg (i.e., the reference values for Lp and Ps at 0 °C, respectively) together with Lp and Ps at 4, 15, and 25 °C. (D) The activation energies for water and CPA (EG and PG) transport across the cell membrane. (E) The membrane permeability coefficients (Lp and Ps) and their temperature dependence determined by this study together with those reported in the literature25, 27, 41–46.