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Fertilization of human in-vitro matured oocytes for both the fresh (control) and the perfused (subjected to a CPA addition-removal cycle) groups by intracytoplasmic sperm injection (ICSI, A) and photomicrographs (B) showing the morphology of the oocytes before fertilization and 2-pronuclei (2PN) and 4-cell embryos developed from the fertilized oocytes.
