Fig. 1.

Identification of T. brucei clathrin-associated proteins. Clathrin and its associated proteins were immunoprecipitated from cryomilled powder generated from GFP-tagged CHC (CHC::GFP) bloodstream- or procyclic-stage cells. (A) Coomassie-stained SDS-PAGE shows bands specific to anti-GFP affinity isolates versus controls, confirmed as known CHC-associated proteins by western blot and MALDI-TOF MS. (BSF, bloodstream-form parasites; PCF, procyclic-form parasites) (B) Domain organisation of selected high-confidence clathrin-associated proteins identified by LC-ESI MS (see Table 1 and Table S1). Domain predictions were generated via HHPRED, HMMER and InterProScan.