Fig. 4.

Heterologous expression of TbCAP::GFP fusions. (A) C-terminal GFP fusions were constructed for full-length TbCAP141 and TbCAP80, and truncated proteins bearing the entire predicted N-terminal globular domain and the predicted β-rich extreme N-terminus alone. (B) Expression of TbCAP full-length and truncation constructs in bloodstream-form parasites under the control of tetracycline. Low level expression necessitated amplification of native GFP fluorescence by staining with anti-GFP followed by fluorescent secondary antibodies. Scale bar: 5 μm. (C) Expression of TbCAP full-length and truncation constructs in COS-7 cells. Scale bar: 20 μm.