Fig. 7.

Interactome subtending CME in trypanosomes. Whereas clathrin is distributed widely throughout the trypanosome endomembrane system, flagellar pocket-specific factors act in the earliest stages of endocytosis. TbCALM and TbEpsinR are likely recruited via localised lipid accumulation. TbCAP141 and TbCAP80 are also enriched in flagellar pocket-associated clathrin structures. We propose that the large disordered regions of these proteins likely contribute to the generation of local membrane curvature through molecular crowding. Additionally, a conserved core of clathrin-associated accessory proteins (TbVps45, TbAAKL and TbHsc70) likely act to coordinate clathrin coat dynamics and regulate the sorting of the conserved SNARE machinery.