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. 2016 Nov 28;45(6):3519–3527. doi: 10.1093/nar/gkw1154

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© The Author(s) 2016. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com

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Figure 5. — VLP–RNAi_Let-7 inhibits Pan-Ras expression and demonstrates in vitro cytotoxicity in U87 cells. (A) Western blot analysis of Pan-Ras levels in U87 cells incubated for 48 h under various conditions. Lane 1: no treatment (negative control). Lane 2: Let-7 siRNA delivered by transfection with lipofectamine (positive control). Lane 3: VLP-RNAi_Let-7. Lane 4: VLP_WT (negative control). Lane 5: VLP-RNAi_GFP (negative control). (B) Cell viability U87 cells treated with VLP–RNAi_Let-7 and VLP–RNAi_GFP. Cell viability was determined after treatment with various concentrations of VLP–RNAi_Let-7 for 24 h () and 48 h () and VLP–RNAi_GFP for 24 h (▪) and 48 h (). The data are reported as percentage survival. Values are expressed as mean ± SD (n = 8).

Inline graphic — VLP–RNAi_Let-7 inhibits Pan-Ras expression and demonstrates in vitro cytotoxicity in U87 cells. (A) Western blot analysis of Pan-Ras levels in U87 cells incubated for 48 h under various conditions. Lane 1: no treatment (negative control). Lane 2: Let-7 siRNA delivered by transfection with lipofectamine (positive control). Lane 3: VLP-RNAi_Let-7. Lane 4: VLP_WT (negative control). Lane 5: VLP-RNAi_GFP (negative control). (B) Cell viability U87 cells treated with VLP–RNAi_Let-7 and VLP–RNAi_GFP. Cell viability was determined after treatment with various concentrations of VLP–RNAi_Let-7 for 24 h () and 48 h () and VLP–RNAi_GFP for 24 h (▪) and 48 h (). The data are reported as percentage survival. Values are expressed as mean ± SD (n = 8).