Table 2. The Sau1 RM systems with published recognition sequences.
| Strain name and genome reference | Clonal Complex or Sequence Type | S subunit name in REBASE | Recognition sequence | TRDs assigned | Suggested generic name | Experimental method | Reference for target specificity and method |
|---|---|---|---|---|---|---|---|
| MW2 (53) | CC1 | S.SauMW2I | CCAY-5-TTAA | AF | CC1-1 | g, s, a | g (31) |
| S.SauMW2II | CCAY-6-TGT | AG | CC1-2 (CC8-2) | g, s, a | a (36) | ||
| N315 (54) | CC5 | S.SauN315II | CCAY-6-GTA | AH | CC5-2 | g, s, a | s (CC8-1 and CC8-2 in strain NRS384 are from ref. 35) |
| S.SauN315I | AGG-5-GAT | BD | CC5-1 (CC8-1) | g, s, a | |||
| MRSA252 (55) | CC30 | S.SauMRSII | GWAG-5-GAT | CD | CC30-1 | g, s | s (35) g, s (this work) |
| S.SauMRSI | GGA-7-TCG | JK | CC30-2 | s | s (35) s (this work) | ||
| JKD6159 (56) | CC93 | S.SauJKDIII | GAAG-5-TAC or complement | Not a Sau1 system | CC93-3 | s | s (35) |
| S.SauJKDII | GGHA-7-TCG | b*K | CC93-2 | s | Note the ambiguity in assigning CC93-1 and CC93-3 is clarified with strains ED133 and 32320 and from Table 3. | ||
| S.SauJKDI | CAG-6-TTC | Ma* | CC93-1 | s | |||
| ED133 (57) | CC133 | S.Sau133ORF451P | CAG-5-RTGA | ME | CC133-1 | g | g (36) |
| S.Sau133ORF1794P | GGA-7-TTRG | Jd* | CC133-2 | s | s (this work) | ||
| 32320 (58) | CC133 | S.Sau32320ORFAP | CAG-5-RTGA | ME | CC133-1 | g | g (36) |
| S0385 (59) | CC398 | S.SauSTORF499P | ACC-5-RTGA | NE | CC398-1 | g, s | g (36) s (this work) |
Target sites are shown from 5΄ to 3΄ with the length of the non-specific spacer shown as a number. Underlined A or T indicates the site of adenine methylation on the top or bottom strands respectively. The experimental methods used are indicated as g = target obtained by DNA cleavage with a purified enzyme, s = target obtained by SMRT sequencing of E. coli ER2796 genomic DNA, a = target obtained by ATPase assay with a purified enzyme. Full details are given in the Supplementary Data. S.Sau133ORF1794P is characterized in this work but is included here as it is part of the RM system found in strain ED133. SauMRSI and SauMRSII characterized by Monk et al. and S.SauSTORF499P characterized by Chen et al. are also further characterized in this work.