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. 2017 Apr 21;14:13. doi: 10.1186/s12989-017-0193-5

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© The Author(s). 2017

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 3 — Safe design of MOx by surface passivation. a Formation of EDTMP-metal conjugate on MOx surface, b Ion release and oxidative capability of uncoated and EDTMP coated MOx, and c Comparison of ROS generation and cell viabilities in BEAS-2B cells exposed to uncoated and EDTMP coated MOx. The measurement of ion release and oxidative capability was described in the legend of Fig. 1. After 16 h exposure of BEAS-2B cells to 100 μg/mL MO suspensions, intracellular ROS generation was visualized by Mitosox staining. The cell viabilities were determined by MTS assay in BEAS-2B cells after 24 h incubation with 200 μg/mL MO suspensions. * p < 0.05 compared to uncoated MOx