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. 2017 Apr 21;18:317. doi: 10.1186/s12864-017-3684-8

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© The Author(s). 2017

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 1 — Transcriptional Analysis of Fe repressed sRNA NrrF. Iron regulation was confirmed with real time PCR transcriptional analysis of RNA samples isolated for RNA-seq from cells collected 1,2,3,4 and 5 h. a The TSS was resolved by primer extension using a FAM labeled primer yielding a 123 bp fragment as analyzed with Peak Scanner b) and a transcript length of 154 bp as determined by Northern blot analysis d). TSS for NrrF is indicated by an ^ in the NrrF sequence in c), with red denoting the FB sequence and bold type the −10 promoter region. Blue represents the RIT, with the unlined portion of the sequence the location of the FAM labeled primer used for the primer extension c)