Table 1.

Yeast strains used in the study

ID	Species	Information	Source
Brewing strains
P1	S. cerevisiae	VTT-A81062. Maltotriose fermentation, POF+	VTT culture collection
P2	S. cerevisiae	WLP099. No maltotriose fermentation, POF−	White Labs Inc
P3	S. eubayanus	VTT-C12902. No maltotriose fermentation, POF+	VTT culture collection
H1	Hybrid	P1 × P3 interspecific hybrid (VTT-A15225)	Created in this study
H2	Hybrid	P2 × P3 interspecific hybrid	Created in this study
H3	Hybrid	P1 × P2 intraspecific hybrid	Created in this study
T1	Hybrid	H1 × P2 interspecific triple hybrid	Created in this study
T2	Hybrid	Meiotic segregant of T1	Created in this study
Laboratory strains
WT	S. cerevisiae	BY4741 MAT a; his3∆1; leu2∆0; met15∆0; ura3∆0	EUROSCARF (Y00000)
ole1∆	S. cerevisiae	BY4741 MAT a; his3∆1; leu2∆0; met15∆0; ura3∆0; ole1-m2:kanMX	EUROSCARF (Y40963)
erg4∆	S. cerevisiae	BY4741 MAT a; his3∆1; leu2∆0; met15∆0; ura3∆0; YGL012w::kanMX4	EUROSCARF (Y04380)