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. Author manuscript; available in PMC: 2017 Apr 21.
Published in final edited form as: Adv Biol Regul. 2014 Sep 30;57:185–192. doi: 10.1016/j.jbior.2014.09.009

Fig. 1.

Fig. 1

Growth phenotypes for the R64 suppressor of acc1cs. The right panel depicts growth of strains on 2% glucose medium at 30°C (2 days); the middle panel depicts growth on 2% glucose at 16°C (10 days); the right panel depicts growth on 3% glycerol medium (3 days). The red lines divide diploid (2N) and haploid (1N) strains. Key: WT (ACC1+); H-470 (MATα acc1cs); 1341 (MATa acc1cs); R64 (acc1cs pdb1sup). Haploid strains show the tight Csm− phenotype of acc1cs, and the effective suppression of Csm− by the R64 suppressor (middle panel). The R64 × 1341 backcross shows that the R64 suppressor is recessive with respect to Gly−, but dominant with respect to Csm+. The other three diploid strains show that the R64 Gly− phenotype is not complemented when crossed with a pdb1Δ deletion strain, but is complemented with crossed with pda1Δ and kgd1Δ strains. This is compelling genetic evidence that the R64 suppressor is a pdb1 allele.