Figure 4.

PCR-based genotyping of the PFK7 and proCYP98A3 alleles in the sumo1/2^KD line. A and B. True to scale diagrams of the PFK7 and CYP98A3 genes. The amiR-SUMO2 integration sites (arrowheads) are located in (A) PFK7 at +2,531 bp and (B) in CYP98A3 –587 bp, calculated from the start codon (+1). The exons and introns are presented by boxes and broken lines, respectively. The white boxes reflect the 5’- and 3’-untranslated regions, while the black boxes refer to the coding regions. The primers used for genotyping are indicated by small black arrows with their ID numbers given (not to scale; see also Table 1). The orientation of the amiR-SUMO2 constructs is indicated using gray-dash arrows (from the Left Border to the Right Border). (C) PCR-based genotyping using the primers represented in (A) and (B) of the SUMO2^KD line B and sumo1/2^KD lines B21 and B22#1, 2 lines obtain from the same cross between sumo1–1 and SUMO2^KD line B. See also Table 1 for primer sequences.