Figure 3.

Effect of PEG-induced drought on FdTSPO transcript abundance. Samples for quantitative real-time reverse transcription PCR (qRT-PCR) were taken before (0 h) and after (3 h, 6 h, 24 h) addition of 20% PEG6000 (w/v) in (A) green light (GL) and (B) red light (RL) to assess FdTSPO transcript accumulation in response to induced stress. Relative expression level compared with ORF10B gene is shown as average values ( ± SD, n = 3). Expression of each FdTSPO is shown relative to expression levels in wild-type F. diplosiphon cells at time 0 h in the respective light condition. Asterisks indicate a significant difference (*p < 0.01, **p < 0.05) between expression level at 0 h relative to each time point after treatment with PEG, as determined by ANOVA and Tukey test.