Figure 6.

Complementation of mutated RPS4 (N195D or Y950H) to Arabidopsis rps4-21 mutants. The rps4-21/RPS4-Ws^N195D -#10, and -#11, and rps4-21/RPS4-Ws^Y950H -#2, and -#3 lines represent independent transgenic rps4-21 plants harboring the genomic RPS4-Ws^N195D and RPS4-Ws^Y950H fragments, respectively. (A) Quantification of C. higginsianum in planta by qRT-PCR. Twenty eight-day-old plants were spray-inoculated with C. higginsianum. The inoculated leaves were harvested at 5 dpi and total RNA was isolated. QRT-PCR was performed with Ch-ACT primers for each sample. (B) Quantification of Pst-avrRps4 in planta. The leaves of 5-week-old plants were infiltrated with bacterial suspensions (5 × 10⁴ cfu ml⁻¹). The leaves were harvested at 3 days after inoculation. Bacterial growth (cfu cm⁻²) was assessed using five leaf disks by cell counting. Bars indicate SE. The asterisks indicate statistical significance from the Ws-2 WT controls (Dunnett's method,²⁸ P < 0.01). This experiment was repeated at least two times with similar results.