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Cho1p shows specificity for l-serine. The PS synthase assay was performed with 0.5 mM [3H]-serine and 0.1 mM CDP-DAG. An excess of d-serine (A) or l-threonine (B) was added at varying concentrations. Only d-serine or l-threonine at concentrations 400-fold that of l-serine show any inhibition in [3H]-PS production. Values are shown as an average of three experiments averaged and normalized to the wild-type control. P = 0.0361.
