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Dose-dependent killing of S. aureus USA300 and SH1000 biofilms by 5HE-C14-TMA. Bacterial biofilms were grown in plasma-coated glass-bottom MaTeKTM dishes in RPMI-1640 for 24 h at 37 °C. 5HE-C14-TMA was added at a range of concentrations and incubated for a further 24 h. Biofilm bacterial viability was evaluated using live/dead imaging (top panels), quantified using ImageJ and presented as mean fluorescence intensity (±SD) (left histogram, USA300; right histogram, SH1000).
