Table 1. Level of (c)mnm⁵s²U in tRNAs of various strains used in this study.

Strain	Relevant genotype	s2C/Ψ 254 nm	mnm5s2U/Ψ a 254 nm	s2U/ Ψ 254 nm	(c)mnm5s2U/s4U 314 nm	s2U/s4U 314 nm
GT7321	mnmA+, mnmE+	0.10	0.042	<0.001	0.047	<0.001
GT8173	mnmA16<>cat	0.12	<0.001	<0.001	<0.003	<0.002
GT8176	mnmE17<>kan	0.12	<0.003	0.048 b	<0.002	0.019
GT8177	pmnmA+/ mnmA16<>cat c	0.12	0.054	<0.001	0.046	<0.002
GT7440	mnmA3 (G24D)	0.12	< 0.001	d	< 0.001	D
GT7453	tusB27 (Q31stop)	0.12	< 0.001	d	< 0.001	D
GT7432	tusE30 (K128stop)	0.11	< 0.003	d	< 0.002	D
GT7436	mnmE13 (codon 241–271 deleted)	0.14	< 0.001	d	< 0.001	D
GT7478	mnmG1(gidA1, 58 nt deletion from A402)	0.13	<0.002	d	< 0.001	D

a) The cmnm5s2U peak is hidden under contaminating material, so it is not possible to determine the area.

b) This level is likely to be an overestimate due to poor separation from especially G. (See Fig 4C). The level is about 100% of the average level of mnm⁵s²U in the two wild type strains GT7321 and GT8177.

c) Strain GT8177 contains a plasmid from the Saka collection,; [45], which harbors the wild type allele of mnmA⁺ gene and the mnmA16<>cat insertion on the chromosome.

d) These analysis were made using a Supelco C-18 column, which does not separate s²U from s⁴U. However, this analysis determines excellently the level of mnm⁵s²U at 254 nm and both cmnm⁵s²U and mnm⁵s²U at 314 nm.