Table 4. The double mutant mnmA, mnmE is nonviable on rich medium at 15 to 37°C.
| Temp (°C) | Donor (Relevant genotype) | Recipient (Relevant genotype) | TcR No. of colonies (days) | KmR No. of colonies (days) |
|---|---|---|---|---|
| 37 | GT8176(mnmE17<>Km, STM3453-2550::Tn10dTet) | GT7321(mnmA+mnmE+) | 976 (3) | 691 (3) |
| 37 | “ | GT8177 (pmnmA+/ mnmA16<>cat) | 463 (3) | 393 (3) |
| 37 | ” | GT8173 (mnmA16<>cat) | 915 (3) | 0 (7); 2a |
| 30 | ” | GT7321(mnmA+mnmE+) | 584 (3) | 308 (5) |
| 30 | ” | GT8173 (mnmA16<>cat) | 628 (3) | 0 (29) |
| RT | ” | GT7321(mnmA+mnmE+) | 521 (4) | 191 (14) |
| RT | ” | GT8173 (mnmA16<>cat) | 482 (6) | 0 (35) |
| 15 | ” | GT7321(mnmA+mnmE+) | 155 (10) | 28 (10) |
| 15 | ” | GT8177 (pmnmA+/ mnmA16<>cat) | 224 (10) | 80 (10) |
| 15 | ” | GT8173 (mnmA16<>cat) | 0 (44) | 0 (44) |
Recipient cells were grown in rich medium at 37°C. Following transduction using phage P22 grown on indicated donor strains, selection was made on rich plates containing either Km (50 ug/ml) or Tc (20ug/ml). Selection for TcR colonies shows the efficiency of transduction. The plates were incubated at indicated temperatures for indicated days (in parenthesis) when the number of colonies was scored. Plasmid pmnmA+ is the pNTR-SD mnmA+ plasmid harbouring the wild type allele of the mnmA+ gene from E. coli [45].
a) Two small colonies were observed after seven days but they were not analysed further due to their instability and poor growth.