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. 2017 Apr 21;12(4):e0175929. doi: 10.1371/journal.pone.0175929

Fig 5. Effects of RNA-associated proteins on the cryptic exon of GLA transcripts.

Fig 5

(A-C) Virus-mediated shRNA knockdown of various genes as indicated. SMYD2 and NSD1 are histone methyltransferases which preferentially methylates Lys-36 of histone H3. (D) RNA-chromatin immunoprecipitations (RNA-ChIP) analysis on the cryptic exon area in Int4 of GLA was performed using antibodies against SF2/ASF and SRp20 with IgG as a control. (E) Co-immunoprecipitation results using the indicated antibodies for immunoprecipitation and analyzing by western blotting. FD cells, Fabry disease cells; NC: negative control; WB, western blot; SMYD2, SET and MYND domain containing 2; NSD1, nuclear receptor binding SET domain protein 1.