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. 2017 Apr 21;12(4):e0175929. doi: 10.1371/journal.pone.0175929

Fig 6. Effects of amiloride on the regulation of GLA (IVS4 + 919G>A) splicing.

Fig 6

Cells were treated with different concentrations of amiloride for 24 hours and then harvested for RT-PCR analysis (A), or Western blot analysis (B,D). Actin and Histone H3 were used as internal standards. (C) The result of enzyme activity assay from FD cells after the treatment with or without amiloride for 24 hours. Data were presented as the mean ± standard deviation from three independent experiments. Asterisk represents significant difference (p-value < 0.05). (E) RT-PCR and Western blot results from cells pretreated with (+) or without (-) okadaic acid and then exposed to amiloride for 24 hours. FD cells, Fabry disease cells; Amil, amiloride; OA, okadaic acid.