Table 3. Primers for real-time quantitative PCR for lipid related genes and β-actin of black seabream (Acanthopagrus schlegelii).
| Gene | Nucleotide sequence (5’ –3’) | Size (bp) | GenBank reference or Publication |
|---|---|---|---|
| accα 1 | F: AGTAGCCTGATTCGTTGGT | 154 | KX066238 |
| R: GATTGAGGAGTCTGTTCGC | |||
| 6pgd 2 | F: GAAGGGCTTGCTGTTTGTT | 101 | KX066237 |
| R: GTGGCCAGGCCTCTATATG | |||
| g6pd 3 | F: TCTCGCCAAGAAGAAAATC | 297 | KX078573 |
| R: GCCAGGTAGAACAAACGGT | |||
| fas 4 | F: AAGAGCAGGGAGTGTTCGC | 213 | KX066240 |
| R: TGACGTGGTATTCAGCCGA | |||
| srebp-15 | F: TGGGGGTAGGAGTGAGTAG | 247 | KX066235 |
| R: GTGAAGGGTCAGTGTTGGA | |||
| lpl6 | F: CTGCTACTCCTCTGCCCA | 204 | KX078571 |
| R: ACATCCCTGTTACCGTCC | |||
| cpala 7 | F: TGCTCCTACACACTATTCCCA | 203 | KX078572 |
| R: CATCTGCTGCTCTATCTCCCG | |||
| atgl8 | F: GCATCCAGTTCACCCTCAC | 241 | KX078570 |
| R: TTTGCCTCATCTTCATCGC | |||
| hsl9 | F: AGCAACTAAGCCCTCCCCATC | 179 | KX066236 |
| R: TCTTCACCCAGTCCGACACAC | |||
| pparα 10 | F: ACGACGCTTTCCTCTTCCC | 183 | KX066234 |
| R: GCCTCCCCCTGGTTTATTC | |||
| elovl5 11 | F: TCGTACTTCGGTGCCTCCCT | 176 | KU372149 |
| R: GGCCATATGACTGCAAATATTGTC | |||
| fads2 12 | F: AGCCAGGACCGAAATAAAA | 113 | KX058437 |
| R: AGGTGGAGGCAGAAGAACA | |||
| β-actin | F: ACCCAGATCATGTTCGAGACC | 212 | Jiao et al. [50] |
| R: ATGAGGTAGTCTGTGAGGTCG | AY491380 |
1 accα, acetyl-CoA carboxylase alpha;
2 6pgd, 6-phosphogluconate dehydrogenase;
3 g6pd, glucose 6-phosphate dehydrogenase;
4 fas, fatty acid synthase;
5 srebp-1, sterol regulator element-binding protein-1;
6 lpl, lipoprotein lipase;
7 cpt1a, carnitine palmitoyltransferase 1A;
8 atgl, adipose triglyceride lipase;
9 hsl, hormone-sensitive lipase;
10 pparα, peroxisome proliferators-activated receptor alpha;
11 elovl5, elongase of very long-chain fatty acids 5;
12 fads2, fatty acyl desaturase 2.