Table 8. Serum and liver oxidation and antioxidant parameters of juvenile black seabream (Acanthopagrus schlegelii) fed different dietary DHA/EPA ratios.
| Parameter | Dietary DHA/EPA ratio | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 0.65 | 1.16 | 1.60 | 2.03 | 2.67 | ANOVA P | F-value | ||||||
| Mean | SEM | Mean | SEM | Mean | SEM | Mean | SEM | Mean | SEM | |||
| Serum | ||||||||||||
| GSH-PX (units/ml) | 23.61 | 1.21 | 25.91 | 0.80 | 23.75 | 0.20 | 23.31 | 1.69 | 22.93 | 1.27 | 0.63 | 0.68 |
| SOD (units/ml) | 77.48 | 4.14 | 81.51 | 4.15 | 91.96 | 8.00 | 85.37 | 6.48 | 83.69 | 3.89 | 0.49 | 0.92 |
| GSH (mg GSH/L) | 39.57 | 2.89 | 38.57 | 1.62 | 41.90 | 1.59 | 36.33 | 4.47 | 37.65 | 6.01 | 0.86 | 0.32 |
| MDA (nmol/ml) | 10.38a | 1.36 | 10.38a | 0.89 | 10.69a | 0.98 | 15.50b | 0.49 | 15.07b | 0.28 | 0.00 | 8.90 |
| Liver | ||||||||||||
| GSH-PX (units/mg protein) | 181.43 | 13.81 | 203.68 | 16.25 | 232.30 | 12.94 | 218.65 | 17.09 | 207.02 | 7.96 | 0.20 | 1.83 |
| SOD (units/mg protein) | 77.92 | 3.84 | 70.37 | 4.55 | 82.25 | 7.57 | 76.41 | 4.42 | 75.38 | 3.53 | 0.59 | 0.74 |
| GSH (mg GSH/g protein) | 9.54 | 0.71 | 9.37 | 0.67 | 9.02 | 0.31 | 9.65 | 0.66 | 10.03 | 0.09 | 0.76 | 0.46 |
| MDA (nmol/mg protein) | 1.85 | 0.16 | 1.95 | 0.11 | 1.98 | 0.10 | 1.98 | 0.07 | 2.51 | 0.25 | 0.08 | 2.88 |
Data are reported as means and SEM (n = 3). Values in the same line with different superscripts are significantly different (P < 0.05).
GSH, glutathione; GSH-PX, glutathione peroxidase; MDA, malondialdehyde; SOD, superoxide dismutase.