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. 2017 Apr 21;12(4):e0176156. doi: 10.1371/journal.pone.0176156

Fig 3. Plasma corticosterone at induction.

Fig 3

Plasma corticosterone levels throughout induction were measured every third day. (a) Rats (n = 12/group) were bled prior to treatment (T-60) for baseline measures. Corticosterone levels at baseline decreased over the 7-day induction period. (b) After 30min habituation to the chamber half the animals were injected with RU486 and half with vehicle. Animals were then bled 30min later (T0). At this time point RU486 was shown to increase plasma corticosterone levels compared to vehicle groups across all days. A main effect of Day was also observed at this time point. (c) Immediately after being bled at T0 rats were injected with MK-801 (or saline) and a final blood sample was collected 60min later (T60). At the T60 time point on Day 1 there was a main effect of both RU486 and MK-801. A post-hoc Dunnett’s test on the repeated measures ANOVA indicate that the RU486+MK-801 group had significantly elevated plasma corticosterone levels across all days. *p<0.05 main effect of Day; # p<0.05 main effect RU486 pre-treatment; ^ p<0.05 main effect MK-801 drug treatment; @ p<0.01 RU486+MK-801 vs Vehicle+Saline. Data are presented as the mean ± SEM.