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. 2017 Apr 7;13(4):e1006703. doi: 10.1371/journal.pgen.1006703

Fig 1. Triplin acts through ethylene signaling pathway to cause a triple response phenotype in Arabidopsis seedlings.

Fig 1

(A) Phenotypes of 3-day-old, dark-grown Col-0 seedlings treated with 100 μM triplin, 50 μM ACC, or 1% (v/v) DMSO as a control. Scale bar represents 1 mm. (B) The chemical structure of triplin. (C) The hypocotyl length of 3-day-old, dark-grown Col-0, etr1-1, etr1-2, ein2-5 and ein3 eil1 seedlings treated with 100 μM triplin, 50 μM ACC, or 1% (v/v) DMSO as a control. Each experiment was repeated three times, more than 30 seedlings were used every time. Error bars represent SEM. ***P < 0.0001 (two-tailed Student’s t-test) indicated a significant difference of the hypocotyl length of the mutants compared to Col-0 treated with 100 μM triplin. (D) qRT-PCR analysis the expression of the ethylene response gene ERF1 treated by 1% (v/v) DMSO, 100 μM triplin, or 50 μM ACC. Each experiment was repeated three times, and error bars represent SEM.