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. 2017 Apr 7;13(4):e1006703. doi: 10.1371/journal.pgen.1006703

Fig 2. ran1-1 and ran1-2 are hypersensitive to triplin and copper can partially reverse the effects of triplin.

Fig 2

(A) Phenotypes of 3-day-old, dark-grown seedlings of Col-0, ran1-1 and ran1-2 treated with 10 μM triplin. (B) Triplin dose responses of Col-0, ran1-1, ran1-2 and two 35S:RAN1-GFP (ran1-2) transgenic lines. Data is the average hypocotyl length under each condition. Each experiment was repeated three times, more than 30 seedlings were used every time. Error bars represent SEM. (C) Phenotypes of 3-day-old, dark-grown seedlings of ran1-2, ran1-2 etr1-1 and ran1-2 ein2-5 treated with 100 μM triplin. (D) The phenotypes of 3-day-old, dark-grown seedlings of Col-0 treated with 100 μM triplin in the presence of 20 μM ZnSO4, 20 μM CuSO4 or H2O as a control. (E) The hypocotyl length of seedlings as described in (D). Each experiment was repeated three times, more than 30 seedlings were used every time. Error bars represent SEM. **p < 0.01 indicated the difference of the hypocotyl length between the seedlings treated with CuSO4 compared with H2O and ZnSO4 in the presence of 100 μM triplin. Scale bars represent 1 mm.