Fig. 1.

WGBS reveals extensive variation of DNA methylation in insects. (A) Genomic levels of DNA methylation in insects ranges from zero (all Diptera examined) to ∼14% (Blattella asahinai). Higher ranges are observed for coding regions; zero (all Diptera examined) to ∼42% (Blattella germanica). Overall, levels are highest in Blattodea, and do not always associate with social species. (B) A species tree constructed from nuclear and mitochondrial loci, which was used in Phylogenetic Generalized Least Squares (PGLS) analysis. Results from this analysis revealed that there is no correlation between social behavior and DNA methylation (table 1). Values at nodes are posterior probabilities <0.95; all blank nodes have ≥0.95 posterior probability.