Fig. 3.

Characteristics of age-dependent lipidome changes. (A) Yearly amplitude of lipidome changes calculated as the ratio of concentration changes occurring within a one-year interval to the concentration changes occurring across the lifespan in human brains based on concentrations of the 7,589 age-dependent lipids. The points represent the mean change amplitude. The area shows the variance of the change amplitude estimate (±1 SD). The lighter shades of colors indicate younger ages. The dark red numbers show the percentage of concentration changes occurring during the first 20 years of life and during 20–60 years interval. (B) Amplitude of cumulative concentration changes in adult brains relative to infant brains based on lipids of the corresponding lipid class in humans. The data are shown for the top 10 most abundant lipid classes jointly constituting 70% of the human brain lipidome and the remaining lipids (others). The numbers next to bars show percentage of the human brain lipidome contributed by the lipid class. (C) The proportion of white matter in the human samples estimated using quadratic programming and simulated annealing. The circles filled with lighter shades of color indicate younger ages. The slope and P-value were calculated using the linear regression model fitted to the white matter proportions.