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. 2016 Dec 24;34(3):640–653. doi: 10.1093/molbev/msw263

Fig. 5.

Fig. 5

The evolutionary innovations of functional WOX5 with apical stem-cell maintenance activity and intercellular mobility in Arabidopsis roots. (A) Lateral view of the root apical meristem (RAM) showing the seed plant WOX5-GFP proteins (left and middle panels) driven by the native WOX5 promoter, were detected in DSCs. However, the fern CrWUL-GFP protein (right panel) as driven by the native WOX5 promoter, was restricted to the QC. Blue arrow, QC position; Yellow arrow, DSC layer. (B) In contrast to the pWOX5::AtWOX5-GFP and pWOX5::AtWOX5-GFP constructs, the pWOX5::CrWUL-GFP construct was unable to rescue the wox5-1 aborted DSC. (C) In pWOX5::Mini-CrWUL-GFP plants, the GFP signals were detected in the whole root stem-cell niche, including the DSCs. (D) The pWOX5::Mini-CrWUL-GFP construct was able to rescue wox5-1 root defects. (E) Schematic representation of Arabidopsis root stem-cell niche maintenance. The red line outlines the root stem-cell niche. Modified from Sarkar et al. (2007). (F) In pRGF1::CrWUL-GFP plants, the GFP signals were simultaneously detected in the QC and DSCs. (G) The pRGF1::CrWUL-GFP construct was able to rescue the wox5-1 defects. Scale bars, 20 µm.