Table 1.
Characteristics of 21 microsatellite primers developed for Stipa breviflora.
| Locus | Primer sequences (5′–3′) | Repeat motif | Allele size (bp) | Ta (°C) | Fluorescent dye | GenBank accession no. | Putative function [Organism] | E-value |
| SB13 | F: CTTCTTGCGAGTACAGCGATTT | (TC)8 | 137 | 57 | HEX | KY355614 | PREDICTED: plasminogen activator inhibitor 1 RNA-binding protein-like [Oryza brachyantha] | 4.00E-171 |
| R: CAAACAGAGCTCAACATCACAAA | ||||||||
| SB16 | F: CAGTGGTTTTTGTTTAACAGCAG | (CAA)6 | 148 | 51 | TAMRA | KY355615 | Predicted protein [Hordeum vulgare subsp. vulgare] | 1.00E-83 |
| R: GCCCGTACCATAATTTTCTTTTT | ||||||||
| SB21 | F: ATGTACTTGGAAGAAACGAAGCA | (CGG)6 | 94 | 57 | FAM | KY355616 | Predicted protein [Hordeum vulgare subsp. vulgare] | 6.00E-13 |
| R: TGCTGTTGTGATCTACAGGTTTG | ||||||||
| SB32 | F: CAATTGTAGAGGGGTAACAACGA | (TTC)5 | 153 | 57 | TAMRA | KY355617 | Predicted protein [Hordeum vulgare subsp. vulgare] | 3.00E-102 |
| R: AGTCAGTGTGCTGCTGTCAAATA | ||||||||
| SB35 | F: CTACTGACATCCAACGTATTGAA | (G)16 | 153 | 57 | TAMRA | KY355618 | Uncharacterized protein LOC100845604 [Brachypodium distachyon] | 2.00E-34 |
| R: GAGATCAGGTTTACGAACCCC | ||||||||
| SB40 | F: GATCGCCATTGGTAGTATGTAAA | (AG)9 | 160 | 57 | FAM | KY355619 | — | — |
| R: TTCCTTCTTCATCCTTCCACTTG | ||||||||
| SB42 | F: TCCCTCAGAGAAAAATCAAAACA | (CT)7 | 138 | 56 | HEX | KY355620 | Uncharacterized protein LOC100826478 [Brachypodium distachyon] | 3.00E-24 |
| R: ATCATCCTGTACACCGTCGTCTA | ||||||||
| SB43 | F: AAATCCTTCCTCGCGCTC | (ACC)5 | 152 | 60 | TAMRA | KY355621 | Uncharacterized protein LOC101756208 [Setaria italica] | 9.00E-100 |
| R: CTCATCGATCTCCTCGCTTCT | ||||||||
| SB45 | F: CCGACACACACAAGATGAGC | (GCG)5 | 111 | 51 | FAM | KY355622 | Uncharacterized protein LOC100823613 [Brachypodium distachyon] | 3.00E-53 |
| R: GCTGGTGCAGGACCTCCC | ||||||||
| SB46 | F: TCCTTCTCTGTATATAAAGCCCG | (CT)8 | 136 | 56 | HEX | KY355623 | PREDICTED: NAC domain-containing protein 78 [Brachypodium distachyon] | 0 |
| R: ATGCATTTGCCTGGAATGTT | ||||||||
| SB49 | F: ACTCTCCTGCAACTCTGTGAAAG | (GA)9 | 144 | 56 | TAMRA | KY355624 | — | — |
| R: TAATGCAAGCATTTGGCTATACA | ||||||||
| SB50 | F: AGGAGCATCATCCTTGTCCTC | (GAG)6 | 134 | 57 | HEX | KY355625 | PREDICTED: paramyosin-like isoform X1 [Brachypodium distachyon] | 2.00E-122 |
| R: ACCGCACTTATCTCCTCTTTCTT | ||||||||
| SB52 | F: AGAAGAAGAGGAAGAAGAACCCA | (GGC)6 | 146 | 57 | TAMRA | KY355626 | Uncharacterized protein LOC101770650 [Setaria italica] | 5.00E-78 |
| R: AGATCCACCGCTCTTCCTAGT | ||||||||
| SB53 | F: GCAAAGGAACCTACGTCTTCC | (GGC)6 | 133 | 59 | HEX | KY355627 | PREDICTED: cysteine-rich receptor-like protein kinase 10 [Brachypodium distachyon] | 2.00E-147 |
| R: GAGAGGCTCATATGGCTGAAC | ||||||||
| SB54 | F: CACAAGGTACCGAAAAGGAAAG | (GA)6 | 90 | 59 | FAM | KY355628 | Hypothetical protein EUGRSUZ_C01942, partial [Eucalyptus grandis] | 5.00E-11 |
| R: ACCAACCCACTCTCTCTCTCTCT | ||||||||
| SB55 | F: AAATCTGCTCTCAGGTGGAATC | (TGCGAT)4 | 131 | 54 | HEX | KY355629 | PREDICTED: heat shock protein 82 [Brachypodium distachyon] | 2.00E-13 |
| R: AAATCAATCGCACTCGCAAT | ||||||||
| SB57 | F: AACTTGTGAAGGTTTGCAATGTC | (AT)10 | 148 | 57 | TAMRA | KY355630 | Predicted protein [Hordeum vulgare subsp. vulgare] | 1.00E-37 |
| R: AACCCAGTCACCTCTGACAACTA | ||||||||
| SB77 | F: ACTTTATTCCGCATGCTA | (AAG)5 | 125 | 56 | FAM | KY355631 | Predicted protein [Hordeum vulgare subsp. vulgare] | 0 |
| R: TTCGTTCTTTTGTCTGTG | ||||||||
| SB78 | F: TCACCATTACCCATTCGCTTCCT | (GCG)5TTG(GA)10 | 191 | 60 | FAM | KY355632 | PREDICTED: ATP synthase subunit d, mitochondrial-like [Setaria italica] | 6.00E-88 |
| R: TCATCTTCGGATCTCCTCCTCCC | ||||||||
| SB79 | F: GATGGTCCACTCATCCAGGCTGT | (TC)9 | 235 | 54 | FAM | KY355633 | RecName: Full = Thioredoxin H-type; Short = Trx-H; AltName: Full = TrxTa [Triticum aestivum] | 6.00E-54 |
| R: GTGCGTGAGAAAGAAGCGGTCCT | ||||||||
| SB81 | F: CGCTCCACTACCTTTCGTATCAC | (CT)11(GT)8 | 194 | 60 | FAM | KY355634 | Uncharacterized protein LOC100845363 [Brachypodium distachyon] | 9.00E-137 |
| R: GGAATGAATGCCTTGAGTGAGTC |
Note: Ta = annealing temperature.