Table 1.
Characteristics of 46 microsatellite loci developed for Smilax aspera.
| Locus | Primer sequences (5′–3′) | Repeat motif | Allele size (bp) | Ta (°C) | A | GenBank accession no. |
| S003 | F: TCCCCATTTCTCCTCACTTG | (TTTTC)5 | 100 | 53 | 4 | KY358008 |
| R: GCCACTACAACAACTTAGTGATTTTG | ||||||
| S004 | F: GCCCACTTTCATTGCCTTTA | (TCA)8 | 111 | 53 | 15 | KY358009 |
| R: AATGTGGGCGTGGTAAAAAG | ||||||
| S006 | F: AAAGGGGATGAGGAGAAGGA | (AAG)7 | 133 | 59 | 9 | KY358010 |
| R: AAACCACCATGACTCCTCCA | ||||||
| S007 | F: CTGCTTCCAGACAGAGGAGG | (TGGTT)5 | 139 | 59 | 8 | KY358011 |
| R: ACACTTCTTGGGTTGGCATC | ||||||
| S009 | F: GAGTGAGGAGGGAGGAGCTT | (TC)33 | 159 | 58 | 23 | KY358012 |
| R: CCGGAGAACCAGATGAAGAC | ||||||
| S016 | F: AGAACTTGAGGGTGTGTGGG | (T)10(TC)6 | 230 | 58 | 16 | KY358013 |
| R: TTCATGCATACTTTTGCCGA | ||||||
| S028 | F: TAATCCCTCGCGAAATCAAG | (GATC)5 | 120 | 53 | 3 | KY358014 |
| R: CCCAAAATCGATCGAGAAAA | ||||||
| S030 | F: AAGCCAAGCAAACCCATTTA | (GA)14 | 126 | 59 | 15 | KY358015 |
| R: CACCCTCTGACTCCGAAGAG | ||||||
| S034 | F: CAGGGAGTTGGTCCTCAAAA | (T)21 | 154 | 59 | 12 | KY358016 |
| R: ATGGTTGCAAAGAAACACCC | ||||||
| S046 | F: CTAAGGCGATATCCTCAGCG | (GTGGGC)5 | 226 | 59 | 7 | KY358017 |
| R: CAGCCACTTGGTATCCACCT | ||||||
| S049 | F: AAGGGACATTTTTGTTCCCC | (TAAA)6 | 248 | 59 | 4 | KY358018 |
| R: GCAAGTTAAGCAACACAGTTAAGG | ||||||
| S052 | F: AGATCCACAGTTCCACCTGC | (AAACTAT)10 | 266 | 59 | 8 | KY358019 |
| R: GCGCTTGATGTGCTCAAATA | ||||||
| S053 | F: GATCTGGGTTTCTCGTTGGA | (CTGGGA)5 | 269 | 59 | 6 | KY358020 |
| R: GGCCATTTGGAAGAGACTGA | ||||||
| S057 | F: GAGATTTCCAGCAAAACCCA | (CGAG)4 | 291 | 58 | 5 | KY358021 |
| R: AGTTTCTGGGCCCTCTGTCT | ||||||
| S060 | F: CCATGGTGGACGACTTTCTT | (GAT)6 | 311 | 59 | 3 | KY358022 |
| R: GCATGGAAACGCCTATGATT | ||||||
| S062 | F: CTTGGCAACACCAATCAATG | (TCCT)7 | 326 | 59 | 9 | KY358023 |
| R: TGCACGTGATCACTGGATCT | ||||||
| S063 | F: CATTTCGATGAATCGTGTGG | (CATCT)5(TC)23 | 332 | 59 | 21 | KY358024 |
| R: GTAGGGTTCGGTGCTGATGT | ||||||
| S066 | F: TCGATTTCCACCCATTTCTC | (CGCCAC)5 | 354 | 59 | 10 | KY358025 |
| R: GCTGAGTACTTGAGGGCGTC | ||||||
| S072 | F: CAGTGCCTCTTCCTTGCTTC | (TGG)5(GTGGCC)3 | 402 | 59 | 16 | KY358026 |
| R: TATACCCAGGTCTCCGAACG | ||||||
| S081 | F: ATTTCGCCACTACCTTGCAC | (CCCT)6 | 103 | 50 | 8 | KY358027 |
| R: ATCCTTCATTCAATGCCGAG | ||||||
| S083 | F: GGACTGGATTCCGTTTTGCT | (CCTCTA)4 | 105 | 50 | 4 | KY358028 |
| R: AGCCAGGACATTGCCTTTAC | ||||||
| S085 | F: TGTTGGGTGAGCAAAACAAA | (T)16 | 109 | 53 | 16 | KY358029 |
| R: ACCTTTCTCCCCACTTGCTT | ||||||
| S086 | F: TAATTGGCTTCGGATTGACC | (AG)9 | 112 | 50 | 28 | KY358030 |
| R: GGAATTCGTTCTTCCCCATT | ||||||
| S087 | F: GGACTTGGTCATCAGGTCGT | (TC)12TAGGTC(TCGGA)3 | 116 | 55 | 21 | KY358031 |
| R: TTGTGCAACCAAACTCCAGA | ||||||
| S089 | F: CACAAGCTTGATGAGGTCCA | (TGGTT)3 | 125 | 53 | 7 | KY358032 |
| R: AAGGACACGGACCATGAAAG | ||||||
| S090 | F: AGCAGCCTTGGGCTTATTTT | (TAAAC)3 | 132 | 53 | 5 | KY358033 |
| R: TTCTGTTGTGCGGATATTGG | ||||||
| S093 | F: GAAGGGAGGGAGGAGAAGTG | (AG)12 | 135 | 53 | 7 | KY358034 |
| R: CCGTTTAAAGATCCCGTCAA | ||||||
| S094 | F: TGCTGGAAGAACAACGACTG | (GCTGTT)4 | 143 | 50 | 4 | KY358035 |
| R: GTTACCGTTGGTCACCTGCT | ||||||
| S096 | F: TGGATTCATGTGTTTGGCTG | (A)22 | 145 | 55 | 8 | KY358036 |
| R: AAATCAGGCCTCCTCATTGTAA | ||||||
| S097 | F: CACCTTCTCCTCCTCTTCCC | (TTC)8 | 148 | 51 | 9 | KY358037 |
| R: TCATCTCCCCTCTTCTTCCC | ||||||
| S100 | F: CTGGAGATCTCACCCTCTCG | (CCCTCT)3 | 155 | 50 | 6 | KY358038 |
| R: CAATGAGACAGTCCGGATCA | ||||||
| S104 | F: AATTGGGATTTGATGATCGC | (TC)17 | 168 | 53 | 11 | KY358039 |
| R: CCAAAAACCCACGAGAGAAA | ||||||
| S105 | F: GCTGGTACTTCTTCTTGCCG | (GGCGGA)3 | 168 | 55 | 6 | KY358040 |
| R: ACTTCGAGAACAGCCTCCAA | ||||||
| S110 | F: TCACGTGTGAGGTTCTAGCG | (AG)7AA(AG)14 | 181 | 59 | 3 | KY358041 |
| R: TGGCGTCCCAGTGAGTGT | ||||||
| S113 | F: ACGTAACTCTCGGTGCCATC | (AG)11 | 185 | 55 | 5 | KY358042 |
| R: CGTGTGGAAGGGAGGTAAAA | ||||||
| S116 | F: ATGACATCCCCTCCCTCTCT | (TC)9 | 191 | 55 | 15 | KY358043 |
| R: CCCCACCATTGTCTTGAAGT | ||||||
| S120 | F: AGGCCAAGACTATCAGCGAA | (GTG)7 | 204 | 53 | 3 | KY358045 |
| R: TCTTTCTTGCTCCAGGCATT | ||||||
| S121 | F: GGGAACACTACCTTCTGCCA | (CGATCT)4 | 211 | 61 | 3 | KY358046 |
| R: TTGAGATCTGGGGAGGTTTG | ||||||
| S122 | F: TGTGGTGCTTGATGAGCTTC | (CTG)7 | 214 | 50 | 3 | KY358047 |
| R: CGTTGCACAGAGCGAATAAA | ||||||
| S126 | F: CTTCTCCGCATACCACCTGT | (CT)10 | 227 | 53 | 6 | KY358048 |
| R: GCTCTGCGTCTGTTCCATTT | ||||||
| S130 | F: ATGCTTGACACGCTTGATTG | (TGC)8 | 247 | 53 | 12 | KY358049 |
| R: AGCTGCTTGGACAGCAAAAT | ||||||
| S132 | F: ACGGTCTCTTTCAAGAAGGG | (AG)12 | 251 | 55 | 11 | KY358050 |
| R: GATGAAGGAGAACGCAAAGC | ||||||
| S134 | F: GAGAGCCCACGTGAAGTGAT | (GA)15 | 258 | 55 | 27 | KY358051 |
| R: CCCCATAAATGTGGGAGATG | ||||||
| S139 | F: GCAAAGCTCTTCTCCTCCCT | (TTC)5 | 282 | 50 | 7 | KY358052 |
| R: CTGGATGGCTTTGGATAGGA | ||||||
| S144 | F: GACCCCATGGATACGAGAAC | (GGGGTC)3 | 306 | 55 | 4 | KY358053 |
| R: CTAAACCCGACTCCCCAAAT | ||||||
| S148 | F: AGAACCAGCAGAGCGACATT | (CAG)7 | 350 | 55 | 4 | KY358054 |
| R: TTGCGTCAGCTTACCCTTCT |
Note: A = number of alleles per locus; Ta = optimized annealing temperature.