Figure 4. Mad2l1 inactivation in mouse embryonic fibroblasts fully alleviates the spindle assembly checkpoint.
(A) Recombination efficiency measured by genomic PCR (top) and Western blot (bottom) showing partial to complete Mad2l1 deletion in MEFs following retroviral doxycycline-inducible Cre. Actin serves as loading control. (B) Average phospho-Histone H3 staining of dox-inducible Cre-transduced MEFs following 6 hr of nocodazole treatment. Error bars show the SEM of at least two biological replicates. (C) Average mitotic index of thymocytes isolated from Paclitaxel or control-injected mice 4–6 hr post-treatment. Error bars show SEM of at least four biological replicates.