Table 1.
Summary of phenotype screens in this study.
| Screen | Outcomes | Points of screen | Related figure and/or dataset |
|---|---|---|---|
| Essential gene | * 257 B. subtilis essential genes required for growth in LB at 37 °C were defined. * Genome-wide essential gene screen by constructing deletion mutants using two different markers simultaneously. |
Detailed procedure for defining essential genes. List of B. subtilis essential genes. Condition-essentiality of 30 genes. Discussion of essentiality changes from this study. Comparison of essential genes in B. subtilis and other bacteria. |
Table S3, tab A legend Table S3, tab A; Figure 2A Table S3, tab A Table S3, tab B Table S3, tab C; Figure 2D |
| Growth phenotype | * Genome-wide relative fitness of libraries in different growth conditions. | List of relative fitness (growth phenotypes) of mutants in all conditions. | Table S4, tab B |
| Cold-sensitive (cs) phenotype | * Genes required for growth in LB at 16 °C were identified. * Data suggested that overexpression of some prophage genes are toxic in low temperature. |
List of cold-sensitive mutants and discussion about their phenotypes. Functional groups enriched in cs phenotype. |
Table S4, tab C Figure S3E |
| Auxotrophic gene | * Genome-wide auxotroph screen identified 98 auxotrophic genes in B. subtilis. | List of B. subtilis auxotrophic genes. Comparison of auxotrophic genes in B. subtilis and E. coli. |
Table S4, tab D Table S4, tab E; Figure 3B |
| Utilization of carbon (C) or nitrogen (N) source | * Genes required for utilizing specific C and N sources were identified. * Several pathway connections in B. subtilis metabolism were clarified. |
Genes required for utilization of specific C or N source. Analysis of pathways for C or N source utilization in B. subtiils. |
Table S4, tab F Figure S4 |
| Double mutant analysis | Identification of genes performing the final step of Phe and Tyr biosynthesis. | Methodology and analysis of linkage effect. New genes required for Phe and Tyr biosynthesis. |
Figure 5 Figure S5 |
| Competence | Genome-wide competence gene screen identified competence genes in B. subtilis. | List of competence defective mutants. Transformability of previously known competence defective mutants in this study. Conservation of competence genes in other bacteria. |
Table S5, tab B Table S5, tab C Table S5, tab D; Figure S6 |
| Sporulation | Genome-wide sporulation screen using colorimetric method confirmed known sporulation mutants and identified new players. | List of relative sporulation scores of mutants. List of sporulation defective mutants. Functional groups enriched in sporulation phenotype. Conservation of sporulation genes in other bacteria. |
Table S6, tab A Table S6, tab B Figure 6E Table S6, tab E; Figure S7C |