REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
Chemicals, Peptides, and Recombinant Proteins
Lysogeny broth (LB), Lennox	Fisher scientific	Cat# BP1427-2
Tryptic Soy Agar (TSA)	BD Biosciences	Cat# 236950
Nutrient broth	BD Biosciences	Cat# 234000
Agar	BD Biosciences	Cat# 214030
Hemin	Sigma-Aldrich	Cat# 51280
Yeast extract	BD Biosciences	Cat# 212750
Casamino acids	BD Biosciences	Cat# 223050
Potassium phosphate monobasic	Sigma-Aldrich	Cat# P0662
Potassium phosphate dibasic	Sigma-Aldrich	Cat# P8281
Trisodium citrate dihydrate	Fisher scientific	Cat# S279-500
Ferric ammonium citrate	Sigma-Aldrich	Cat# F5879
Potassium glutamate monohydrate	Sigma-Aldrich	Cat# G1501
Potassium aspartate	Sigma-Aldrich	Cat# A6558
Manganese chloride tetrahydrate	Sigma-Aldrich	Cat# M3634
Magnesium sulfate heptahydrate	Sigma-Aldrich	Cat# M1880
L-tryptophan	Fisher scientific	Cat# BP395-100
Dextrose (D-[+]-glucose)	Sigma-Aldrich	Cat# D9434
IPTG	Denville scientific	Cat# C18280-13
MOPS	Sigma-Aldrich	Cat# M1254
Potassium sulfate	Sigma-Aldrich	Cat# P9458
Ammonium Chloride	Sigma-Aldrich	Cat# A9434
L-alanine	Sigma-Aldrich	Cat# A7627
D-alanine	Sigma-Aldrich	Cat# A7377
L-arginine	Sigma-Aldrich	Cat# A8094
L-glutamine	Sigma-Aldrich	Cat# G3126
Glycine	Sigma-Aldrich	Cat# G7126
L-histidine	Sigma-Aldrich	Cat# H8125
L-isoleucine	Sigma-Aldrich	Cat# I2752
L-leucine	Sigma-Aldrich	Cat# L8000
L-lysine	Sigma-Aldrich	Cat# L5626
L-methionine	Sigma-Aldrich	Cat# M9625
L-proline	Sigma-Aldrich	Cat# P0380
L-serine	Sigma-Aldrich	Cat# S4500
L-threonine	Sigma-Aldrich	Cat# T8625
L-valine	Sigma-Aldrich	Cat# V0500
L-cysteine hydrochloride monohydrate	Sigma-Aldrich	Cat# C7880
L-phenylalanine	Sigma-Aldrich	Cat# P2126
L-tyrosine	Sigma-Aldrich	Cat# T3754
p-amino benzoic acid	Sigma-Aldrich	Cat# A9878
p-hydroxy benzoic acid	Sigma-Aldrich	Cat# H5376
2,6 -diaminopimelic acid	Sigma-Aldrich	Cat# D1377
L-Homoserine	Sigma-Aldrich	Cat# H6515
Shikimic acid	Sigma-Aldrich	Cat# S5375
Adenine	Sigma-Aldrich	Cat# A8626
Cytosine	Sigma-Aldrich	Cat# C3506
Guanine	Sigma-Aldrich	Cat# G11950
Uracil	Sigma-Aldrich	Cat# U0750
Biotin	Sigma-Aldrich	Cat# B4501
Nicotinamide	Sigma-Aldrich	Cat# N3376
(−)-Riboflavin	Sigma-Aldrich	Cat# R9504
Thiamine hydrochloride	Sigma-Aldrich	Cat# T4625
Calcium pantothenate	Sigma-Aldrich	Cat# C8731
Pyridoxine hydrochloride	Sigma-Aldrich	Cat# P6280
Vitamine B12	Sigma-Aldrich	Cat# V2876
Succinic acid	Sigma-Aldrich	Cat# S3674
D-(−)-Fructose	Sigma-Aldrich	Cat# F0127
L-(−)-malic acid sodium salt	Sigma-Aldrich	Cat# M1125
Glycerol	Fisher scientific	Cat# G30-4
Sodium pyruvate	Sigma-Aldrich	Cat# P2256
D-gluconic acid	Sigma-Aldrich	Cat# G9005
L-ornithine	Sigma-Aldrich	Cat# O2375
Potassium nitrate	Sigma-Aldrich	Cat# P8394
Calcium chloride dihydrate	Sigma-Aldrich	Cat# C3881
Zinc chloride	Sigma-Aldrich	Cat# 208086
Cobalt(II) chloride hexahydrate	Sigma-Aldrich	Cat# 60820
Sodium molybdate dihydrate	Sigma-Aldrich	Cat# 71756
Copper(II) chloride dihydrate	Sigma-Aldrich	Cat# 307483
Ferric chloride hexahydrate	Sigma-Aldrich	Cat# F2877
2,2′-dipyridyl	Sigma-Aldrich	Cat# D216305
Kanamycin sulfate	Sigma-Aldrich	Cat# K1377
Erythromycin	Sigma-Aldrich	Cat# E5389
Lincomycin hydrochloride	Sigma-Aldrich	Cat# L2774
Spectinomycin dihydrochloride pentahydrate	Sigma-Aldrich	Cat# S9007
Ampicillin sodium salt	Sigma-Aldrich	Cat# A9518
SalI-HF (restriction enzyme)	New England Biolabs	Cat# R3138
HindIII-HF (restriction enzyme)	New England Biolabs	Cat# R3104
NheI-HF (restriction enzyme)	New England Biolabs	Cat# R3131
SphI-HF (restriction enzyme)	New England Biolabs	Cat# R3182
Phusion hot-start DNA polymerase	Thermo scientific	Cat# F540L
Q5 High-Fidelity DNA polymerase	New England Biolabs	Cat# M0491L
T4 DNA ligase	New England Biolabs	M0202
T4 DNA polymerase	New England Biolabs	M0203
DNA polymerase I, Large (Klenow) Fragment	New England Biolabs	M0210
T4 Polynucleotide kinase	New England Biolabs	M0201
Klenow fragment (3′→ 5′ exo-)	New England Biolabs	M0212
dATP solution	New England Biolabs	N0440
dNTP solution mix	New England Biolabs	N0447
Lysozyme (from chicken egg white)	Sigma-Aldrich	Cat# L6876
Triton X-100	Fisher scientific	Cat# BP151-500
Imidazole	Sigma-Aldrich	Cat# I2399
DTT (DL-dithiothreitol)	Sigma-Aldrich	Cat# D9779
HEPES	Sigma-Aldrich	Cat# H3375
Tris(2-carboxyethyl)phosphine hydrochloride, (TCEP)	Sigma-Aldrich	Cat# C4706
2-mercaptoethanol	Sigma-Aldrich	Cat# M3148
Biomol Green	Enzo Life Sciences	Cat# BML AK111
Critical Commercial Assays
Agencourt AMPure XP	Beckman Coulter	Cat# A63881
Dynabeads MyOne Streptavidin C1	Invitrogen	Cat# 65001
QIAquick PCR purification kit	Qiagen	Cat# 28104
DNeasy Blood & Tissue Kit	Qiagen	Cat# 69504
Deposited Data
Experimental Models: Cell Lines
Experimental Models: Organisms/Strains
Bacillus subtilis 168	BGSC	1A1
Bacillus subtilis 168 deletion mutants	This study	BKE or BKK with locus tag numbers
Escherichia coli DH5α	Lab stock	N/A
Escherichia coli Rosetta2 (DE3)	EMD Millipore	Cat# 71400
Escherichia coli BW25113	Baba et al., 2006	N/A
Escherichia coli BW25113 ΔserB	Baba et al., 2006	N/A
Recombinant DNA
pDR240a	This study	N/A
pDR242a	This study	N/A
pDR244	This study	N/A
pDR110a	Rudner lab	N/A
pET28a	EMD Millipore	Cat# 69864
Sequence-Based Reagents
Primers used in this study are listed in Table S1.	This study	N/A
Software and Algorithms
Primer3	Untergasser et al., 2012	http://biotools.umassmed.edu/bioapps/primer3_www.cgi
Bowtie2	Langmead and Salzberg, 2012	http://bowtie-bio.sourceforge.net/bowtie2/index.shtml
Iris	Kritikos et al., submitted	https://github.com/critichu/Iris/
Protein BLAST	Altschul et al., 1990	https://blast.ncbi.nlm.nih.gov/Blast.cgi
GSEA	Broad Institute	http://software.broadinstitute.org/gsea
iTOL	Letunic and Bork, 2016	http://itol.embl.de/
Other