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. 2017 Feb 9;8(13):20588–20601. doi: 10.18632/oncotarget.15246

Figure 2. ZFP36L1 regulated osteoblastic differentiation of MC3T3-E1 preosteoblasts.

Figure 2

A. RT-qPCR analyses showed the ZFP36L1 expression of ZFP36L1-knockdown (sh36L1) cells in relation to that of control (shEV) cells. B. RT-qPCR analyses. Confluent shEV and sh36L1 cells were induced to undergo osteoblastic differentiation, and the kinetic expression of osteocalcin and osteopontin mRNAs were shown. *, P < 0.05; **, P < 0.01; ***, P < 0.001 versus shEV control at day 0. C. RT-qPCR analyses showed the ZFP36L1 expression of ZFP36L1-overexpressing (ZFP36L1) cells in relation to that of control (EV) cells. D. Quantification of Alizarin Red S stains. Confluent EV and ZFP36L1 cells were induced to undergo osteoblastic differentiation. Cells were stained with Alizarin Red S on days 4 and 14 post-induction. The stains were dissolved and quantitated.