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. 2017 Feb 9;8(13):20588–20601. doi: 10.18632/oncotarget.15246

Figure 7. ZFP36L1 decreased PPARγ2 expression but not Pparγ promoter activity.

Figure 7

ZFP36L1-overexpressing (ZFP36L1) and control (EV) C3H10T1/2 cells were transfected with a luciferase reporter driven by Pparγ promoter and a Renilla control reporter, cells were then induced to undergo adipogenic differentiation, and were harvested at the times indicated. A. RT-qPCR analyses. The expression of Pparγ2 mRNA was examined. Data represent the mean ± S.D. from three experiments. *, P < 0.05; *, P < 0.005 versus control. B. Luciferase assays. Cells harvested after adipogenic induction were analyzed for luciferase and Renilla activities. The normalized luciferase signals of EV and ZFP36L1 cells were shown.