Figure 2. Effect of ³²P on biodistribution of Listeria.

(A) Biodistribution of Listeria. Panc-02 mice were injected intraperitoneal (ip) with 0.5 × 10⁷ CFU of Listeria or Listeria-³²P when tumors were 15 mm (day 14 after tumor cell injection), and 1, 3, and 7 days later the mice were euthanized and analyzed for the number of CFU of Listeria in tumors, metastases (predominantly in pancreas and portal liver, mesenteric lymph nodes along GI) and normal tissues as indicated on the x-axis. The error bars represent the SEM. Representative of 2 experiments. Data showing are the average of from n = 3 mice per group. (B) Biodistribution of ³²P. Panc-02 mice were injected ip with 10⁷ CFU of Listeria-³²P when tumors were 15 mm (day 10 after tumor cell injection), and 1, 4, 24, 48, and 96 hrs later the mice were euthanized and analyzed for radioactive counts (per gram tissue) in tumors, metastases and normal tissues as indicated on the x-axis. n = 5 mice per group. This is the average of one experiment. Mann-Whitney p < 0.05 is significant. All groups were compared to Listeria-³²P. The error bars represent the SEM. ns = not significant. LN = lymph nodes, BM = bone marrow, Met = all metastases combined.