Figure 1. IL-6 is highly expressed in gastric CAFs.
(A, B) The expression of IL-6 in both serum and cancer tissues in gastric cancer patient was detected by ELISA. (C) Frozen tissue sections from gastric cancer and adjacent non-tumor tissue were immunostained by DAPI (nucleus), FITC (IL-6) and PE (α-SMA) (200×). (D) IL-6 protein expression level in the gastric cancer cells (SNU-1, MKN45, SGC7901 and MKN28), gastric CAFs and normal fibroblasts (NFs) was quantified 24hrs after change the culture medium by ELISA. *P < 0.05.