Table 3. Primer sequences used Real-time PCR and Ms-PCR (5′-3′).

	Gene	Forward primer	Reverse primer
Real-time PCR	NSUN2	GAACTTGCCTGGCACACAAAT	TGCTAACAGCTTCTTGACGACTA
	GAPDH	GACTCATGACCACAGTCCATGC	AGAGGCAGGGATGATGTTCTG
Ms-PCR	Unmethylated NSUN2	TGAGTAATTGGGATTATAGATAGGTG	AAAAAATTCATCTAAAAAAAACAAA
	Methylated NSUN2	CGAGTAATTGGGATTATAGATAGGC	AAAAAATTCGTCTAAAAAAAACGAA