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. 2017 Feb 15;8(13):20813–20824. doi: 10.18632/oncotarget.15343

Figure 7. Co-localization of tankyrase1 with γ-tubulin and pericentrin in spindles of multi-centrosomal MDA-MB-231 cancer cells.

Figure 7

Bi-polar co-localization of tankyrase1 and γ-tubulin (A) or tankyrase1 and pericentrin (B) in spindles of untreated MDA-MB-231 cancer cells were identified by confocal microscopy scanning. Fixed cultured MDA-MB-231 cells were immunolabeled for tankyrase1 (green), γ-tubulin (red) and pericentrin (red). Chromosomes are stained by DAPI (blue). After incubation with PJ34 (20 μM, 27 h), the bipolar co-localization of tankyrase1 and γ-tubulin (A) or tankyrase1 and pericentrin (B) turned into multiple scattered foci of the co-localized proteins. Chromosomes were not aligned in the spindle mid-zone in all the PJ34 treated cells. Treatment with ABT888 (n = 20; 20 μM, 27 h) did not impair the bipolar co-localization of tankyrase1 and γ-tubulin (A), or tankyrase and pericentrin (B), nor impaired the chromosomes alignment in the spindle mid-zone in MDA-MB-231 cells. Co-localization of tankyrase1 and pericentrin, or tankyrase1 and γ-tubulin was detected in 95% of the randomly scanned spindles of both treated and untreated cells (n = 20).