Figure 4. Effects of combined RAD001 and AZD6244 treatment on angiogenesis.
(A) HUVEC cells (104 per well) with conditioned medium were seeded on top of Matrigel in 96-well plates for 4 hr. Tube formation was pictured with a bright field microscope. The tube lengths were quantified with the ImageJ software. n.s., not significant. (B) RCC cells were deprived of serum and treated with 0.1 μM RAD001 (RAD), 1 μM AZD6244 (AZD), 0.1 μM RAD/1 μM AZD (Comb), or equivalent volume of DMSO (Ctrl) for 24 hr. VEGF levels in the supernatants were assessed by ELISA. **p < 0.01.