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. 2017 Feb 1;8(13):20881–20894. doi: 10.18632/oncotarget.14930

Figure 6. miR-210-3p directly regulates TWIST1.

Figure 6

A. Luciferase reporter assays using two vectors encoding either putative miR-210-3p binding site in the 3′-UTR of TWIST1 (nucleotides 3058-3064) or after deleting the miR-210-3p binding site. Luminescence intensity was measured in the miR-210-3p transfectants in comparison with the miR-control transfectant. Renilla luciferase values were normalized to firefly luciferase values (* P = 0.0495). B. Luciferase reporter assays using the vector encoding TWIST1 3′-UTR in miR-210-3p-depleted A498 and Caki2 cell lines (* P < 0.0001). C. qRT-PCR data showing that the expression levels of RAD52 and EFNA3, known targets of miR-210-3p, were significantly higher in miR-210-3p-depleted A498 and Caki2 cell lines (* P < 0.0001).