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. 2017 Feb 18;8(13):20961–20973. doi: 10.18632/oncotarget.15468

Figure 2. Afatinib radio-sensitizes HNSCC cells.

Figure 2

(A) SCC1 and SCC10B HNSCC were pretreated with afatinib (0.5 μM) for 24 h and then radiated with 2–8 Gy IR. After 24 h wells were washed and cells were allowed to grow for 2 weeks. Colonies were stained with 1% crystal violet, counted, and survival curves were plotted. (B) SCC1 and SCC10B cells were synchronized overnight in 1% serum containing medium and treated with afatinib alone for 24 h, or combined with 8 Gy IR. After 24 h, cells were fixed and stained with propidium iodide and analyzed by flow cytometry. (C) SCC1 and SCC10B HNSCC were plated on glass cover slips and pre-treated with afatinib (0.5 μM) for 24 h and then irradiated with 8 Gy IR. After 24 h wells were washed and analyzed by confocal microscopy for pγH2AX foci. DNA damage foci were counted and plotted as bar graphs.