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. 2017 Feb 18;8(13):21115–21127. doi: 10.18632/oncotarget.15501

Figure 3. Induction of ER stress by VLX1570.

Figure 3

(A) ALL (MOLT-4 and SUP-B15) and non-ALL (OPM-2 and HeLa) cells were treated with DMSO or different concentrations of VLX1570 for 6 hours. Thapsigargin was used at 10 μM for 6 hrs. Protein lysates were subjected to immunoblotting with antibodies to p-eIF2α, eIF2α, XBP-1s or β-actin. (B) ALL (MOLT-4 and SUP-B15) cells were treated with IC50 concentrations of VLX1570 and/or L-Asp or bortezomib (BZ) for 6 hours. Protein lysates were subjected to immunoblotting with antibodies to p-eIF2α, eIF2α, p-eEF2K or eEF2K. Tunicamycin is an inhibitor of protein glycosylation which in distinction of thapsigargin did not induce p-eIF2α in most of the ALL cell lines (with the exception of Reh, See Supplementary Figure 1). (C) Analysis of polyubiquitin accumulation in RS4;11 cells following exposure to L-Asparaginase.