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. 2017 Feb 9;8(13):21351–21361. doi: 10.18632/oncotarget.15212

Figure 6. Clerosterol affects OCT2 activity in the HEK293-OCT2 cells.

Figure 6

(A) Cisplatin uptake was analyzed by HPLC after the HEK293-OCT2 cells were incubated with clerosterol for 24 h at 37°C, and then incubated with 50 μg/mL of cisplatin for another 4 h at 37°C. (B) OCT2 protein expression in the HEK293-OCT2 cells treated with clerosterol for the indicated period of time, determined by Western blot analysis. GAPDH was used as a loading control. (C) OCT2 mRNA expression was measured by RT-qPCR analysis. Error bars indicate SD. *p < 0.05, **p < 0.01 compared with the control.